MELISA® Laboratory
Danderyds
Hospital
182 88 Danderyd
Sweden |
|
|
|
|
|
|
Patient code:
|
Jeff Clark
|
|
|
|
| Date of sample: |
981022 |
|
|
|
|
| Study: |
9868 |
|
|
|
|
|
Performed tests:
|
Al. Au, Pd, Pb, Cd, Hg, Ag, Ni, PhHg, Cu, TiCl4, Sn, Pt,
MeHg, EtHg, and Sodium lauryl sulfate
|
|
Lymphocyte treatment
|
Cpm |
|
Stimulation Index |
Number of lymphoblasts |
Comments |
| Negative control |
2146 +/- 598 |
|
1 |
2 |
Many activated MF |
|
Lymphocyte treatment
|
Conc. (ug/ml) |
delta Cpm |
Stimulation Index |
Number of lymphoblasts |
Comments |
| PPD |
1 |
6889 |
4,2 |
20 |
|
|
Aluminum
|
40 |
267 |
1,1 |
- |
|
| |
20 |
1591 |
1,7 |
0 |
|
| |
10 |
521 |
1,2 |
- |
|
|
Gold
|
6,25 |
3724 |
2,7 |
9 |
|
| |
3,12 |
1182 |
1,6 |
0 |
Activated MF |
| |
1,56 |
-187 |
0,9 |
- |
|
|
Palladium
|
6,25 |
1055 |
1,5 |
- |
|
| |
3,12 |
1182 |
1,6 |
- |
|
| |
1,56 |
490 |
1,2 |
- |
|
|
Lead
|
25 |
477 |
1,2 |
- |
|
| |
12,5 |
1993 |
1,9 |
- |
|
|
Cadmium
|
3,12 |
-100 |
1,0 |
- |
|
| |
1,56 |
1526 |
1,7 |
- |
|
| |
0,78 |
99 |
1,0 |
- |
|
|
Inorganic Hg
|
0,5 |
6241 |
3,9 |
40 |
|
| |
0,25 |
4122 |
2,9 |
20 |
|
| |
0,125 |
1464 |
1,7 |
- |
|
|
Silver
|
2,5 |
323 |
1,2 |
- |
|
| |
1,25 |
1842 |
1,9 |
- |
|
|
Nickel
|
5 |
22546 |
11,5 |
>100 |
|
| |
2,5 |
40596 |
19,9 |
>100 |
|
| |
1,25 |
5489 |
3,6 |
- |
|
|
Phenyl-Hg
|
0,5 |
3675 |
2,7 |
8 |
Bad slide |
| |
0,25 |
3863 |
2,8 |
15 |
|
| |
0,125 |
3023 |
2,4 |
- |
|
|
Copper
|
0,5 |
-728 |
0,7 |
- |
|
| |
0,25 |
-249 |
0,9 |
- |
|
|
Titanium chloride
|
200 |
-725 |
0,7 |
- |
|
| |
100 |
-659 |
0,7 |
- |
|
| |
50 |
-187 |
0,9 |
- |
|
|
Tin
|
50 |
1551 |
1,7 |
- |
|
| |
25 |
-359 |
0,8 |
- |
|
| |
12,5 |
-946 |
0,6 |
- |
|
|
Platinum
|
25 |
-677 |
0,7 |
- |
|
| |
12,5 |
214 |
1,1 |
- |
|
|
Methyl-Hg
|
0,5 |
-897 |
0,6 |
- |
|
| |
0,25 |
-731 |
0,7 |
- |
|
| |
0,125 |
-340 |
0,8 |
- |
|
|
Ethyl-Hg
|
0,5 |
980 |
1,5 |
- |
|
| |
0,25 |
-1145 |
0,5 |
- |
|
| |
0,125 |
-1079 |
0,5 |
- |
|
|
Sodium lauryl sulfate
|
100 |
640 |
1,3 |
- |
|
| |
50 |
109 |
1,1 |
- |
|
| |
25 |
1680 |
1,8 |
- |
|
|
Footnote:
|
|
|
|
|
|
| CPM |
Counts per minute |
| delta CPM |
Cpm in test cultures minus cpm in neg. control
cultures |
| SI |
Stimulation Index = cpm in test culture divided
by the mean cpm in control cultures |
| PPD |
Tuberculin, purified protein derivative =
positive control |
| - |
Not done |
| MF |
Macrophages |
|
Evaluation:
|
|
|
|
|
|
| Strongly Positive to: |
Nickel |
|
|
|
|
|
Positive to:
|
Inorganic Hg |
|
|
|
|
Weakly positive to:
|
Phenyl-Hg |
|
|
|
|
Negative to:
|
Aluminum
Gold
Palladium
Lead
Cadmium
Silver
Copper
Titanium chloride
Tin
Platinum
Methyl-Hg
Ethyl-Hg
Sodium lauryl sulfate
|
|
|
|
|
Comments
|
Low PPD response |
|
|
|
J.C. comments on PPD: PPD is a good choice for a positive
control in European patients, since there has traditionally been
a TB vaccination program in European countries. In the USA, public
health officials seemed to have decided that TB vaccination was
too risky, or unneeded. I've never been vaccinated for TB, and
to my knowledge have not been exposed to the disease either.
Another member of my group having MELISA tests once lived at
home with a parent who had developed TB. She had even gone through
extensive antibiotic treatments to avoid also developing the
disease. She was the only member of our USA group who didn't
have a low PPD response from MELISA, her PPD scores were: SI=
205, with lymphoblasts > 100.
|