Jeff' Clark's MELISA® Results


MELISA is the registered trademark of MELISA MEDICA FOUNDATION

 MELISA® Laboratory
Danderyds Hospital
182 88 Danderyd
Sweden
         

 

 Patient code:

 

Jeff Clark

     
 Date of sample:  981022        
 Study:  9868        

 

Performed tests:

 

Al. Au, Pd, Pb, Cd, Hg, Ag, Ni, PhHg, Cu, TiCl4, Sn, Pt, MeHg, EtHg, and Sodium lauryl sulfate

 

Lymphocyte treatment

Cpm

Stimulation Index

Number of lymphoblasts

 Comments
Negative control

2146 +/- 598
 

1

2

Many activated MF

 

Lymphocyte treatment

 Conc. (ug/ml)

delta Cpm 

 Stimulation Index

 Number of lymphoblasts

 Comments
 PPD

1

6889

4,2

20
 

 

 Aluminum

 40

267

1,1

 -
 
 

 20

1591

1,7

0
 
 

 10

521

1,2

-
 

 

Gold

6,25

3724

2,7

9
 
 

 3,12

1182

1,6

0

Activated MF
 

 1,56

-187

0,9

-
 

 

Palladium

6,25

1055

1,5

-
 
 

 3,12

1182

1,6

-
 
 

 1,56

490

1,2

-
 

 

 Lead

25

477

1,2

-
 
 

12,5

1993

1,9

-
 

 

Cadmium

3,12

-100

1,0

-
 
 

1,56

1526

1,7

-
 
 

0,78

99

1,0

-
 

 

 Inorganic Hg

0,5

6241

3,9

40
 
 

0,25

4122

2,9

20
 
 

0,125

1464

1,7

-
 

 

 Silver

2,5

323

1,2

-
 
 

1,25

1842

1,9

-
 

 

 Nickel

5

22546

11,5

 >100
 
 

2,5

40596

19,9

>100
 
 

1,25

5489

3,6

-
 

 

 Phenyl-Hg

0,5

3675

2,7

8

Bad slide
 

0,25

3863

2,8

15
 
 

0,125

 3023

2,4

-
 

 

 Copper

0,5

-728

0,7

-
 
 

0,25

-249

0,9

-
 

 

 Titanium chloride

200

-725

0,7

-
 
 

100

-659

0,7

-
 
 

50

-187

0,9

-
 

 

 Tin

50

1551

1,7

-
 
 

25

-359

0,8

-
 
 

 12,5

-946

0,6

-
 

 

 Platinum

25

-677

0,7

-
 
 

12,5

214

1,1

-
 

 

 Methyl-Hg

0,5

-897

0,6

-
 
 

0,25

-731

0,7

-
 
 

0,125

-340

0,8

-
 

 

 Ethyl-Hg

0,5

980

1,5

-
 
 

0,25

-1145

0,5

-
 
 

0,125

-1079

0,5

-
 

 

 Sodium lauryl sulfate

100

640

1,3

-
 
 

50

109

1,1

-
 
 

25

1680

1,8

-
 

 

Footnote:

         
 CPM Counts per minute
 delta CPM Cpm in test cultures minus cpm in neg. control cultures
 SI Stimulation Index = cpm in test culture divided by the mean cpm in control cultures
 PPD Tuberculin, purified protein derivative = positive control
 - Not done
 MF Macrophages

 

 Evaluation:

         
 Strongly Positive to: Nickel        

 

 Positive to:

Inorganic Hg      

 

 Weakly positive to:

Phenyl-Hg      

 

 Negative to:

 

Aluminum
Gold
Palladium
Lead
Cadmium
Silver
Copper
Titanium chloride
Tin
Platinum
Methyl-Hg
Ethyl-Hg
Sodium lauryl sulfate

     

 

 Comments

 Low PPD response      

J.C. comments on PPD: PPD is a good choice for a positive control in European patients, since there has traditionally been a TB vaccination program in European countries. In the USA, public health officials seemed to have decided that TB vaccination was too risky, or unneeded. I've never been vaccinated for TB, and to my knowledge have not been exposed to the disease either. Another member of my group having MELISA tests once lived at home with a parent who had developed TB. She had even gone through extensive antibiotic treatments to avoid also developing the disease. She was the only member of our USA group who didn't have a low PPD response from MELISA, her PPD scores were: SI= 205, with lymphoblasts > 100.