A Call for Four Experiments


 "Candida's Fire" and the medical and science reference pages of this web site form a scientific hypothesis. While the alignment of the data presented is compelling, there is still a need to verify with independent experiments.

Each of the experiments outlined below tie together the main points of how at least some CFS cases are caused by mercury amalgam dental fillings.

Could the medical community researching CFS please take just a momentary pause from the hunt for a virus, and consider fully planned versions of these experiments ?

There are no mystery viruses to be found here, no drugs likely to show up for patenting. It is poison, plain and simple. What you gain by confirming "Candida's Fire" is the privilege of opening the door to prevention and recovery for many many millions of people. It may not make you rich, but it could make you a saint!



Experiment #1: In Vitro Candida Yeast Cultures

Purpose:
Understand Candida albicans and GI effluent in the presence of dissolving dental amalgam and a nutrient stream such as found in a human.

Apparatus: 3 culture dishes with aerator, attached continuous flow tube and collection jars, 3 environmental chambers.
Culture: Candida albicans.
Solutions: Artificial saliva including proteins; Artificial nutrient solution: including starch, sugar, protein, and trace minerals.
Dental Materials: 2 sets of 6, 203Hg marked high copper alloy amalgams 0.5 grams each, set on retrieved juvenile molars each set matched with 2 high noble gold crowns.
Experiment Design: 2 controls and 1 subject.
Control #1: Candida albicans in culture apparatus without dental materials.
Control #2: Culture apparatus with dental materials and no Candida albicans.
Subject: Candida albicans in culture apparatus without dental materials.
Culture Dish Environment: artificial saline maintained with constant aeration, filtered air supply in sterile environmental chamber.
Feeding: On human meal cycle, warmed nutrient solution is washed into and out of dish into flow tube.
Cleaning: After meal cycle culture dish rinsed into flow tube with artificial saline.
Duration: TBD: no less than 3 months, preferably 1 year.
Dental Examinations: Observed corrosion, SEM for surface corrosion type and depth.
Solution examinations: Full account of mercury species in culture dish, flow tube, and collection jars.
Yeast examinations: Full account of mercury species in plasma and cell walls.



Experiment #2: In Vitro Nuetrophil Experiment

Purpose:
Determine effectiveness of control and subject Nuetrophil's against Candida without mercury exposure, and with 2 levels of mercury exposure.
Cultures: Candida albicans retrieved from dish and flow tubes of previous experiment. Nuetrophil's retrieved from test subjects.
Experiment Design: Fully Matrix between cultures, controls and subjects.
Controls: Nuetrophil's from humans with no history of dental amalgams; against: Candida grown without amalgam presence; Candida from culture dish with amalgam; Candida from flow tube with amalgam.
Subjects #1: Nuetrophil's from humans with 5+ years of 6+ dental amalgams, otherwise matching controls, and no complaints of CFS; against: Candida grown without amalgam presence; Candida from culture dish with amalgam; Candida from flow tube with amalgam.
Subjects #2: Nuetrophil's from humans with 5+ years of 6+ dental amalgams, otherwise matching controls, with complaints of CFS; against: Candida grown without amalgam presence; Candida from culture dish with amalgam; Candida from flow tube with amalgam.
Examinations: Double blinded, measure health parameters of donors, then witness effectiveness of Nuetrophil phagocytosis of yeast.




Experiment #3: Mouse Model Experiments

Purpose:
Determine Candida albicans in GI influence on mercury toxokinetics.

Apparatus, methods, etc.: Repeat Jesper Bo Nielsen's inorganic mercuric chloride experiment with following variations.
Experiment Design: Fully Matrix between mice strains, mercury concentrations, control and subject mice.
Controls: Mice without mercury exposure, same lab conditions, chow etc.
Subjects #1: Mice given oral mercury exposures.
Subjects #2: Mice with GI infused with active Candida albicans culture, then given oral mercury exposures.
Subjects #3: Mice with GI sterilized by antibiotics then infused with active Candida albicans culture, then given oral mercury exposures.
Examinations: repeat Nielsen's Toxokinetic study.



Experiment #4: Human Clinical Study

Purpose:
Determine Effectiveness of Candida and mercury detoxification treatments on subjects with CFS.

Experiment Design: Four matched groups of 50 officially diagnosed CFS patients bearing 6+ amalgam fillings; all patient attempt to follow the same yeast free diet; given either: placebos; Candida treatment only; mercury detoxification only; Candida and mercury detoxification treatments.
Duration: 1 year.
Controls: Daily placebos.
Subjects #1: Oral Nystatin powder and acidophilus culture, twice daily.
Subjects #2: Daily 1 gram: NAC, vitamin E; vitamin C; 0.5 grams: glutamic acid; glycine; Full vitamin B complex; RDA for all other vitamins & minerals.
Subjects #3: Oral Nystatin powder and acidophilus culture, twice daily; Daily 1 gram: NAC, vitamin E; vitamin C; 0.5 grams: glutamic acid; glycine; Full vitamin B complex; RDA for all other vitamins & minerals.
Examinations: Measure health parameters at start, then bi-monthly for one year.


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Copyright ©1996 / 1997 Jeff Clark